How to Identify an Unknown GC/MS Peak and Estimate Its Concentration | MSD Data Analysis
An unknown GC/MS peak becomes much more useful once you can determine what it may be and estimate how much is present.
In this video, I show how I work through an unknown peak in MSD data.
I cover:
• Selecting the unknown chromatographic peak
• Reviewing the mass spectrum
• Using a library search for tentative identification
• Evaluating the library match
• Comparing the unknown response to a known compound
• Estimating the unknown compound concentration from the response
Library searching can provide a tentative identification, but the match should still be evaluated together with the rest of the analytical evidence.
I also show a practical way to estimate concentration when the unknown is not part of the original target list.
Vineta Mills Consulting
Practical GC and GC/MS maintenance, troubleshooting, chromatography, software, method development, and laboratory training.
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