How to Run SIM and Scan at the Same Time | Agilent 5977 & 5975 GC/MS Setup
You can acquire SIM and Scan data during the same GC/MS run on Agilent 5977 and 5975 MSD systems.
In this video, I walk through how I set up a SIM/Scan acquisition method and show the differences between the 5977 and 5975 software setup.
I cover:
• Selecting simultaneous SIM and Scan acquisition
• Setting Scan acquisition parameters
• Creating SIM time segments
• Building SIM groups
• Selecting target ions
• Setting dwell times
• Matching SIM windows to chromatographic retention times
• Reviewing differences between the 5977 and 5975 setup
SIM can provide increased sensitivity for selected ions while Scan acquisition still provides full-scan mass spectral information during the run.
For methods containing many analytes, one of the biggest challenges is fitting SIM groups into the chromatographic timeline without interfering with the compounds that still need Scan acquisition.
Vineta Mills Consulting
Practical GC and GC/MS maintenance, troubleshooting, chromatography, software, method development, and laboratory training.
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