How to Use TIC Library Search in Agilent EnviroQuant | NIST GC/MS Identification
Unknown GC/MS peaks can be screened against a mass spectral library to help with tentative identification.
In this video, I show how I use the TIC Library Search function in Agilent EnviroQuant with the NIST mass spectral library.
I cover:
• Opening the TIC Library Search function
• Selecting the NIST library
• Setting search and integration parameters
• Running a library search across the chromatogram
• Reviewing tentative compound identifications
• Comparing match quality
• Reviewing molecular weight, formula, CAS number, and retention time
• Identifying peaks that may need further investigation
Library searching is a useful screening tool, but a library match should be treated as a tentative identification until it is supported by the rest of the analytical evidence.
Vineta Mills Consulting
Practical GC and GC/MS maintenance, troubleshooting, chromatography, software, method development, and laboratory training.
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